Gene expression of brain cells from wild type and Gba flox/flox; Nestin-Cre mice

To investigate neuronopathic Gaucher disease brain cell gene expression, we established Gba flox/flox; Nestin-Cre mice and performed scRNA sequencing of whole brain cells from these and wild type mice. Unbiased clustering analysis revealed that most remarkable change between wild type and Gba flox/flox; Nestin-Cre mice was the appearance of an activated microglia cluster, which exclusively expressed Tnf. Our further study elucidated the mechanism by which activated microglia and TNF contribute to Gaucher disease pathology. Whole brain cells from 2-week-old wild type and Gba flox/flox; Nestin-Cre mice were analyed using scRNA sequencing.