Supplemental Material for Sae-Lee et al., 2020

Figure
S1.
Representative images of degenerating HSN neurons. A-C, Degenerated HSN
neurons had dim (arrow heads) or barely visible (dotted circle) cell bodies. D-G,
Earlier evidence of HSN neuron degeneration was visible as blebbing of the cell
body (arrows). H-I, Processes of
degenerating HSN neurons also showed beading (H, arrows) that was not visible
in healthy HSN neurons (I). Autofluorescence from intestine bright in panels H
and I due to higher gain to visualize HSN axon. VC4 and VC5 neurons fluorescing
out of focus in panels B-F.

Figure
S2. APP::mCherry is expressed throughout
the nervous system. A DIC and fluorescent photomicrographs at 40X along
the length of the worm. A C-terminal mCherry tag shows APP expression. As
expected, we found that APP expressed under a pan-neuronal promoter was seen
throughout the entire nervous system including the nerve ring and along the
ventral nerve cord in the absence (left) and presence (right) of exAPOE4. B Enlarged
photomicrographs of the ventral nerve cord taken mid-body show that APP is
expressed in a punctate pattern in the VCs both when APOE4 expression is absent
(top) and present (bottom). C Enlarged photomicrographs show that APP is
expressed in a punctate pattern in the HSNs.

Figure
S3. APOE3
and APOE4 transgene
levels. A, Semi-quantitative analysis of level of APOE transgene arrays in genomic DNA. Average
band intensities of three independent genomic preparations from five
worms/strain following PCR for human APOE presented as mean ±
SEM. No significant differences were found when comparing levels for APOE3 vs
APOE4 strains after correcting for multiple comparisons. B, Expression
of APOE determined by RT-qPCR of mRNA isolated from 3 (integrated) or 9
(extrachromosomal) near-starved 6-cm plates of worms. DCt
values reveal little difference in gene expression between strains. Values
represent the mean ± SE of two independent
biological replicates with three technical replicates each. Data is represented
as gene expression relative to the reference control gene tba-1.

Table S1. List of strains used
throughout our study arranged by Figure.