Evaluation of r-proteins polypeptide chain elongation rates (amino acids h⁻¹) during exponential growth of <i>M. fortuitum</i>.

(i) and (ii) denote independent cDNA preparations copied from different samples of the same RNA isolate. The variables are defined in Table 1. *, denotes the number of transcripts measured by qRT-PCR per ng of RNA substrate used for cDNA synthesis. Polypeptide chain elongation. Corresponding values of optical density, μ (h−1) and n*c-rrs×10−8 are given in (A). The subscripts (i) and (j) refer respectively to rpsL and rplL. The parameters n*R(i) and n*R(j) were obtained from n*tr(i) and n*tr(j) respectively by multiplying by the factors nR(i)/tr(i) = 2.84 and nR(j)/tr(j) = 2.96 (equation 18). ##, εaa(i) and εaa(j) were evaluated by means of equation (13) where laa(i) = 125 and laa(j) = 131 amino acid residues, remembering that each ribosome has single copy of rpsL (nc-p(i)/R = 1.0) and four copies of rplL (nc-p(j)/R = 4.0).