Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos

The goal of present study is to construct lineage-specific transcriptome to investigate transcriptional changes in the process of apical and basal cell lineage specification. We provide a valuable lineage- specific transcriptome resource to elucidate differential transcription activity underlying progressive divergence of apical and basal cell lineages of early proembryos at genome-wide scale. To investigate spatial and temporary gene expression profile during the process of apical and basal cell lineage specification, proembryos at 1-cell and 32-cell embryo stages were first manually micro-dissected. Then, the apical and basal cell lineages were separated and collected for RNA-seq, respectively. To minimize the variance in the transcriptome and ensure the reliability, three independent biological replicates were performed for each cell type.