Validation of RNA-Seq results by quantitative RT-PCR^a.

aRNA for qRT-PCR validation was obtained from independent biological samples. bDifferential gene expression in WT and ΔsoxR in 3-day old cultures assessed by qRT-PCR. Gene expression was standardized to the housekeeping sigma factor, hrdB, and normalized to WT. cDifferential gene expression in WT and the Δact strain (M511) in 3-day old cultures assessed by qRT-PCR. Gene expression was standardized to the housekeeping sigma factor, hrdB, and normalized to WT. dGenes are arranged in order of increasing SCO number. eThese genes did not demonstrate SoxR- or Act-dependent expression in the qRT-PCR validation assay and were eliminated from further analysis. Validation of RNA-Seq results by quantitative RT-PCRa.