Establishment of hTERT immortalized mouse cardiosphere derived cell line

The analysis showed differences of transcriptomes between primary CDCs and immortalized CDCs as well as revealed TGF-ß inhibitor-SB431542 treatment induced expression changes. We identified hTERT immortalization could result the cell proliferation and cell cycle related genes upregulation in iCDC. KEGG results demonstrated that TGF-ß inhibitor SB431542 treatment could inhibit cardiomyopathy-related pathway which indicate TGF-ß signaling pathway may serve as new target for cardiomyopathy therapy, and iCDC could be used as a heart-related chemicals screening. Overall design: Primary CDCs, iCDCs and SB432542-treated iCDCs mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq2000.