Figure 3. Bifidobacterium strains induce unique changes in local and systemic immune cell distribution and LN architecture.

<strong>Figure 3. </strong><em><strong>Bifidobacterium</strong></em><strong> strains induce unique changes in local and systemic immune cell distribution and LN architecture. A) </strong>Experimental design. C57BL/6 mice treated with antibiotics for 6 days followed by oral gavage with <em>B. pseudolongum</em> ATCC25526 (ATCC), UMB-MBP-01 (MD), or PBS (control). Mice then treated with the immunosuppressant tacrolimus for the next two days. Tissues harvested 2 days after oral gavage of bacteria. Frozen MLN sections stained for <strong>B)</strong> CD11c+ DC, and <strong>C)</strong> F4/80+ MF. Small intestine stained for<strong> ​D) </strong>Foxp3+ Treg, and <strong>E)</strong> CD11c+ DC. LN stained for laminin a4 and laminin a5 with their ratio depicted for <strong>F) </strong>MLN CR, and<strong> G) </strong>PLN CR. MFI values normalized using the sum of mean, and categories compared using one-way ANOVA. * p value &lt; 0.05; ** p value &lt; 0.01, *** p value &lt; 0.001, **** p value &lt; 0.0001. Data representative of 2 separate experiments, 3 mice/group, i.e. 6 total mice per condition over 2 experiments.