NIST-reference fecal samples analyzed following 50;50 MeoH/H20 extraction using Thermofisher Q Exactive. Samples were analyzed in triplicates, including blank and pooled samples as controls.
Mouse serum, brain parenchyma, and brain vessel fractions using methanol extraction for glutamate and glutamine detection. LC-MS/MS was performed in an UltiMate 3000 UPLC system (Thermo Scientific) us