Gene expression profile at single cell level of pancreatic islets cells from mice fed with regular or high fat diet for 6 weeks after weaning.

We have assessed the single-cell transcriptomes of pancreatic islets in a mouse model of obesity and glucose intolerance in order to characterize the cellular and molecular perturbations induced by obesity that lead to T2D. Overall design: C57BL/6J wild-type mice were fed a regular chow diet (RD, N=4) or a 60% high fat diet (HFD, N=5) (Research Diets) for 6 weeks after weaning. Mouse pancreatic islets were isolated by perfusion of the pancreases with CIzyme (VitaCyte) through the common hepatic duct and digested at 37°C for 17 minutes. After gradient-based separation using Histopaque-1077 (Sigma), islets were then hand-picked to avoid exocrine contamination. For details of the analyses, see https://github.com/abcwcm/RubioNavarro_Pancreas