Single-cell profiling of penta- and tetradactyl mouse limb buds identifies early mesenchymal progenitors required to specify digit numbers and asymmetry

Developing limb cells are heterogeneous. We used single cell RNA sequencing to compare a wildtype limb to two different Grem1 tetradactyl mutants at E10.75 and found differences in populations.发育中的肢体细胞具有异质性。我们于胚胎发育第10.75天（E10.75）采用单细胞RNA测序（single cell RNA sequencing）技术，将野生型（wildtype）肢体与两种不同的Grem1四指突变体进行比对，发现二者的细胞群存在差异。