Human first-trimester heart atlas Seurat RDS file for reanalysis

Understanding human cardiac development is essential to improving the diagnosis and treatment of congenital heart defects. This dataset is part of a multi-modal atlas of the developing human fetal heart during the critical first trimester. Using single-nucleus RNA sequencing, we sampled nearly 50,000 cardiac nuclei from three human fetuses at 8.6, 9.0, and 10.7 post-conceptional weeks (pcw). This dataset enabled distinction of 21 cell types, including novel contractile, conductive, and stromal cells. Lymphatic endothelial, epicardial and autonomic neural and glial cells were among the new, smaller populations for which we established high-resolution transcriptional profiles. This is the *.rds file of the cells from all three hearts generated after Seurat-based integration and analysis in R.This dataset is part of a greater project also comprising spatial transcriptomics, anatomy, histology, in situ hybridization and 2D and 3D immunofluorescence, most of which is also present on Figshare. The count matrices and the raw sequencing data for the spatial transcriptomics and this dataset will be available on GEO shortly.This publication is part of the Human Cell Atlas. https://www.humancellatlas.org/publications/