Transcriptome sequencing of drug resistance in Acinetobacter baumannii

Acinetobacter baumannii, a major pathogen causing nosocomial infections, exhibits drug resistance. Efflux pumps are critical in developing multidrug resistance. Berberine hydrochloride exhibits inhibitory effects against bacteria and toxins, demonstrating antibacterial efficacy. However, there is limited research on the berberine hydrochloride combined with multiple antibiotics through regulation of efflux pumps against multidrug-resistant A. baumannii . We evaluated the antibacterial effect of berberine hydrochloride in combination with multiple antibiotics, explored resistance mechanisms regulated by efflux pumps, and analyzed the differential expression of active efflux pump genes before and after berberine hydrochloride exposure, aiming to provide new therapeutic strategies for managing multidrug-resistant A. baumannii infections.