Transcriptional dynamics of iPSC differentiation into ß cells revealed by single-cell RNA sequencing

Induced pluripotent stem cells can be differentiated in vitro into insulin-producing ß cells. In this study we unveil the transcriptional dynamics of the differentiation and explain the heterogeneity of the final cell product, in comparison with ß cells from organ donors. Overall design: Single cell RNAseq of hiPSC during differentiation into pancreatic beta cells and of human islets from organ donors