Phenotypes of C. higginsianum pathogenicity mutants.

* Mutant was generated by transformation with a plasmid other than pPK2. See Text S1.a Wild type data is given 3 days after infection since strong symptom development after 4 days prevented reliable quantification. After 4 days, the number of secondary hyphae ranged from >30 to >60%.1 Visible symptoms after 4 days of spray infection; 0: no visible symptoms. +: reduced symptoms, ++: slightly reduced symptoms, +++: WT.2in vitro appressoria formation on 1,16-hexadecanediol-coated petri dishes in percentage of conidia; 0: 50%.3 Formation of primary hyphae 4 days after spray-infection of A. thaliana Col-0 in percentage of appressoria; 0: 25%.4 Formation of secondary hyphae 4 days after spray-infection of A. thaliana Col-0 in percentage of primary hyphae; 0: ≤ 1%, +: 1–10%, ++: 11–20%, +++: >20%.5 Callose papilla (stained with aniline blue) formed beneath appressoria 3 days after spray-infection of A. thaliana Col-0 in percentage of appressoria; 0: 12%, n.d.: not detectable.6 ROS accumulating cells (stained with 3,3-diaminobenzidine) beneath appressoria 3 days after spray-infection of A. thaliana Col-0 in percentage of total appressoria; 0: 7%, n.d.: not detectable.7 Colony diameter on Czapek-Dox minimal medium after 5 days compared to WT; 0: no growth, +: 30%.Phenotypes of C. higginsianum pathogenicity mutants.