Duration, peak frequency and bandwidth of the USVs produced by different genotypes of male mice.

Duration and peak frequency of USVs were measured automatically according to the protocol introduced in Avisoft SASLab Pro software as described in Materials and Methods. Likewise, the bandwidths of USVs were obtained by measuring the frequency difference of upper and lower bands. A comparison of duration, frequency and bandwidth of USVs between wild-type mice (CD1x129 or C57BL/6) and M2, M4 and M5 muscarinic and D2 dopamine receptor knockout mice was carried out. (Value = Mean��SEM, n = 60 USVs in each group, *p**pt-test).