Exploring diurnal rhythm in Brachypodium distachyon transcriptome through time-course RNAseq

Plants are continuously exposed to varying environmental conditions; some anticipated diurnal changes in light and temperature and others far less predictable. Key to adaptation to a diurnal environment is the anticipation provided by the circadian clock, which coordinates broad changes in gene expression with a period of about 24 h.Here we present the analysis of RNA sequencing to measure transcript abundance over time in Brachypodium distachyon entrained in photo- and thermocycles then transferred to photocycles or thermocycles alone, or constant light and temperature conditions.