Digital Kennison: Kennison deficiency-panel filtered reads for classifying Y-linked sequences of Drosophila melanogaster

Prefiltered Short Reads: Kennison X-Y Translocation Panel========================================================= Prefiltered paired-end Illumina short-read data from the eight strains ofthe Kennison (1981) X-Y translocation panel of Drosophila melanogaster,used by the Digital Kennison pipeline for BLAST-database constructionand read-based coverage validation. Contents--------kl1minus/ — Kennison X-Y translocation strain deficient for kl-1kl2minus/ — Kennison X-Y translocation strain deficient for kl-2kl3minus/ — Kennison X-Y translocation strain deficient for kl-3kl5minus/ — Kennison X-Y translocation strain deficient for kl-5ks1minus/ — Kennison X-Y translocation strain deficient for ks-1ks2minus/ — Kennison X-Y translocation strain deficient for ks-2Yd_Xp_V8/ — Y-deficient / X-plus translocation strain V8Yd_Xp_V24/ — Y-deficient / X-plus translocation strain V24 Each subfolder contains a paired-end read set: <strain>_R1.fq.gz — forward reads <strain>_R2.fq.gz — reverse reads Sequencing platform-------------------Seven strains (all except kl2minus) were sequenced on an Illumina NovaSeq X(10B flow cell), 2x150 bp paired-end, from libraries prepared with theIllumina TruSeq DNA PCR-Free kit (350 bp insert). The kl2minus strain wassequenced on the same platform but from a library prepared with the IlluminaNextera DNA XT kit (tagmentation, variable insert size, modal library size~1100 bp). The kl2minus data therefore have a wider fragment sizedistribution and lower Q30 (~80%) compared with the other seven strains(~92-94% Q30). Prefiltering------------ Raw paired-end Illumina reads were adapter- and quality-trimmed withTrimGalore v0.6.6 (default parameters). To suppress signal from non-Yparalogs during read-based coverage validation, autosomal and X-linkedreads were then removed: trimmed reads were aligned to the Drosophilamelanogaster reference genome R6 (FlyBase release 6.64) with minimap2(-ax sr), and read pairs in which either mate mapped to the majorassembled chromosome arms (2L, 2R, 3L, 3R, 4, X) were discarded. Theremaining reads (those mapping to the Y chromosome, unplaced contigs,or unmapped) constitute the datasets in this archive. Full details of the read processing pipeline are in Uno & Carvalho (2026)and the pipeline source code (https://doi.org/10.5281/zenodo.21271417). Raw reads---------Raw (unfiltered) sequencing reads are archived at NCBI SRA under BioProjectPRJNA1493028 and BioSample accessions: SAMN61545840 : kl1minus (TaxID: 7227) SAMN61545841 : kl2minus (TaxID: 7227) SAMN61545842 : kl3minus (TaxID: 7227) SAMN61545843 : kl5minus (TaxID: 7227) SAMN61545844 : ks1minus (TaxID: 7227) SAMN61545845 : ks2minus (TaxID: 7227) SAMN61545846 : Yd_Xp_V24 (TaxID: 7227) SAMN61545847 : Yd_Xp_V8 (TaxID: 7227) Citation--------If you use these reads, please cite: Uno, F., & Carvalho, A. B. (2026). Digital Kennison: Prefiltered short reads from the Kennison X-Y translocation panel of Drosophila melanogaster. Zenodo. https://doi.org/10.5281/zenodo.21284274 Associated pipeline: Uno, F., & Carvalho, A. B. (2026). Digital Kennison: A bioinformatics pipeline for rapid mapping of sequences in the Drosophila melanogaster Y chromosome. Zenodo. https://doi.org/10.5281/zenodo.21271417 Source code: https://github.com/fabianauno/digital_kennison