Effect of ?Np63 deletion on epigenomic rewiring via differential H3K27ac and H3K27me3 enrichment

Basal cells were isolated from an influenza-infected non-tamoxifen treated Krt5CreERT2; RFP; ?Np63flox/flox mouse and cultured, allowing for inducible ?Np63 deletion in vitro. These basal cells were plated as monolayers and treated with 1uM 4OHT for +4OHT (?Np63 KO) or equivalent volume DMSO for -4OHT (solvent only, ?Np63 WT) for 48 hours. Technical triplicates of ?Np63 WT and ?Np63 KO treated in parallel were harvested for each IP for p63 and each histone modification for ChIP-seq. Overall design: Comparative ChIP-seq of ±?Np63 post-injury basal cell monolayers for H3K27ac, H3K27me3, and p63