Insertion sequence (IS) elements identified within intergenic and flanking regions of serotype A and B strains.

1IS element location with respect to the toxin complex.2Intergenic space (in nucleotides)3IS family, group and homolog identified using the IS Finder database.4The IS element showing greatest homology at the amino acid level using BLAST5Amino acids present/amino acids of the full-length elementIS elements present within the intergenic or flanking regions of the toxin complex from each subtype were identified and characterized by IS family and group. Only partial IS elements ranging from 23–83% homology to the full-length elements, were identified. The IS elements and their location are the same within the BoNT/A1 strains of ATCC 3502, ATCC 19397 and Hall but not shown.