Exosomal microRNA sequencing in Tongxinluo pretreated and non-treated MSC-derived exosomes

In order to investigate the specific mechanisms underlying the cardioprotective effects of Tongxinluo pretreated MSC-derived exosomes (MSCTXL-Exo) in cardiac repair, we performed microRNA sequencing on exosomes secreted from Tongxinluo pretreated MSCs and non-treated MSCs to identify differentially expressed miRNA. We found that 18 miRNAs were identified to be upregulated and 25 miRNAs downregulated (over 2-fold change) in MSCTXL-Exo compared to MSC-Exo. Overall design: Three samples were processed for each kind of exosomes that derived from normal MSCs and TXL pretreating MSCs (MSC-Exo and MSCTXL-Exo). MSCTXL-Exo was isolated from conditioned medium of MSCs pretreated by 400 µg/mL of TXL for 24 hours. RNA isolated from exosomes were analyzed using Illumina Hiseq X Ten platform.