Substitutions in the ASV IN derivatives and their enzymatic activities (crosslinkers placed at residues that are bold).
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The dominant Cys for crosslinking is bolded. 1Processing of viral ends as assayed either by a fluorescence anisotropy release assay or a radioactively labeled processing assay in 10 mM Mg2+ for 30 min. 2Single end joining assessed by a fluorescent joining assay [33]. R244C substitution severely compromises activity in all IN derivatives that contain it.
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2011-12-01
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