Methylated RNA immunoprecipitation sequencing in the Kasumi-1 cell line treated with CoCl2 and echinomycin

To evaluate the role of HIF1a in modulating the m6A abundance, we conducted m6A sequencing in the Kasumi-1 cell line treated with CoCl2 and echinomycin. CoCl2 can mediate the stabilization of HIF1a protein. Echinomycin is a small-molecule inhibitor of HIF1a, which functions through sequence-specific binding to HRE to competitively inhibit HIF1a binding to its target genes. The m6A sequencing results of the Kasumi-1 cell line treated with CoCl2 and non-treated control were uploaded previously (GSE168778). The differential m6A peaks analysis between CoCl2 + echinomycin group and CoCl2 group was performed. Overall design: Kasumi-1 cells were treated with 100 uM CoCl2 and 10 nM echinomycin for 24 hours. A total of 3 biological replicates were performed.