Date for Effects of Fluid Shear Stress Duration on the Mechanical Properties of HeLa Cells using Atomic Force Microscopy

This study aims to investigate the effects of fluid shear stress duration on the mechanical properties of HeLa cells, thereby elucidating the mechanical interactions between cancer cells and the fluid environment during tumor metastasis. To this end, we established an in vitro fluid shear stress cell experimental system and analyzed the flow field characteristics within a parallel plate flow chamber using computational fluid dynamics (CFD) software, ensuring the stability of laminar flow and the uniformity of shear stress distribution. Subsequently, we employed atomic force microscopy (AFM) to measure the mechanical properties of HeLa cells at different time points under a physiological fluid shear stress of 10 dyn/cm². The experimental results revealed that with increasing duration of fluid shear stress exposure, significant changes occurred in the morphology, height, and Young’s modulus of HeLa cells, indicating that fluid shear stress has a substantial impact on the mechanical properties of cancer cells. These data provide new insights into the behavior of cancer cells within the in vivo fluid environment and offer important theoretical foundations for biomedical engineering research.This dataset comprehensively documents the effects of fluid shear stress duration on the mechanical properties of HeLa cells, aiming to reveal how varying durations of fluid shear stress alter cellular mechanical characteristics and their potential roles in tumor metastasis. The dataset consists of four main components: Atomic Force Microscopy (AFM) experimental results, Computational Fluid Dynamics (CFD) simulation results, three-dimensional models of the parallel-plate flow chamber system, and statistical analyses of cell mechanical properties. The AFM results include cell morphology images and Young’s modulus maps under five experimental conditions (0, 5, 15, 30, and 60 minutes) at 10 dyn/cm² shear stress, analyzed using NanoScope Analysis 1.9. The CFD results, simulated using ANSYS Fluent 18.0, describe the flow field characteristics under four shear stress conditions (5, 10, 15, and 20 dyn/cm²). The parallel-plate flow chamber system, designed in Solidworks 2022, includes detailed 3D models and manufacturing drawings. The statistical results, presented in an Excel file, summarize the measured Young’s modulus of HeLa cells, providing quantitative insights into the impact of fluid shear stress on cellular mechanics. The dataset can be analyzed using tools such as NanoScope 1.9 Analysis for AFM data, ANSYS Fluent 18.0 for CFD results, Solidworks 2022 for 3D models, and Microsoft Excel for statistical analysis.