Bacterial wilt pathogen induced spatial shifts of root-associated microbiome and metabolome of potatoes

Plant root-associated microbiomes play an important role in plant health, yet their responses to bacterial wilt infection remain unclear. This study investigated spatial variations in microbiome and metabolome composition across three root-associated niches, root surrounding soil, rhizosphere, and endosphere, of healthy and Ralstonia solanacearum-infected potato plants. Microbial diversity was analyzed using full-length 16S rRNA and ITS sequencing, and metabolic profiling was conducted using LC-QTOF-MS.