Hedgehog signaling affects platelet-derived growth factor signaling mediated alveolar septum formation during postnatal lung development [scRNA-seq]

HH inhibition reproduces the lung phenotype of PDGF pathway knockout lungs and results in profound effects on differentiation and gene expression of Pdgfra-expressing alveolar mesenchymal cells, in particular myofibroblasts. Whole lung single cell suspensions of Gli1creERT2/+;R26RtdTom/+ reporter lungs (P6) from mice, labelled with tamoxifen at P1 followed by treatment with anti-HH antibody 5E1 or control IgG at P3 were loaded onto a GEM code instrument (10xGenomics).