BRD4 promotes gastric cancer progression and metastasis through acetylation-dependent stabilization of Snail

The puropose of this study is to reveal a noncanonical post-transcriptional regulatory function of BRD4 that maintains cancer growth and dissemination, with immediate translational implication for treating metastatic malignancies with clinically available bromodomain inhibitors. The next-generation RNA sequencing was used to confirm the transcriptom overlapping change after BRD4 and Snail knockout separately. Overall design: mRNA profiles of gastric cancer cells which were generated by depletion of control, Snail and BRD4 were generated by deep sequencing, in triplicate, using Illumina hiseqxten .