five

small RNA sequencing for CRISPR array processing

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NIAID Data Ecosystem2026-05-02 收录
下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP144980
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We developed an efficient, one-step method for constructing large CRISPR arrays. Three assembled CRISPR arrays of FnCas12a were subjected to small RNA sequencing after co-incubation with FnCas12a encoding plasmid in TXTL. The purpose of the NGS is to examine if the generated array can be processed correctly into individual crRNA.
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2025-01-02
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