Organ-Specific Effects of Siesta Deprivation and Caffeine on ER and Mitochondrial Stress in Mice

This study investigates the effects of siesta deprivation (SD), both independently and in combination with caffeine administration (SD+Cf), on molecular functions in mice. Male ICR mice were randomly divided into three groups: a control group with no interventions, an SD group, and an SD+Cf group. Following a two-week intervention, frontal cortex samples were collected at Zeitgeber time 0 (ZT0) and Zeitgeber time 12 (ZT12) for RNA-seq analysis. This research seeks to elucidate the molecular consequences of siesta disruption and caffeine consumption, providing insights into their impact on brain function.