Maximum binding capacities (BMAX) of [3H]NMS binding to the membranes of the cells treated with xanomeline, carbachol, oxotremorine or pilocarpine are expressed as pmol of binding sites per mg of membrane protein.

Intact cells were exposed to 10 µM xanomeline for 1, 3 or 10 min or for 10 min to 1 µM carbachol, 1 µM oxotremorine or 3 µM pilocarpine or sham-treated (control) and washed with KHB for 10 min or 1 hour and then membranes were prepared as described in Methods. *, different from control, a, different from shorter treatment with xanomeline, b, different from 10-min washing, PFile S1.