BaRTv2: A highly resolved barley reference transcriptome for accurate transcript-specific RNA-seq quantification

BaRTv2 represents a new and improved Reference Transcript Dataset built upon a broad framework of highly informative Pacbio Iso-seq full length transcripts incorporating accurate SJ, TSS and TES information. It is based upon the European 2-row spring barley cv. Barke which itself is highly relevant to contemporary barley agriculture and current research. The combination of the novel Iso-seq analysis and filtering pipeline, the new automated quality control pipeline for short read assembled transcripts and detailed transcript characterisation and annotation make BaRTv2 a high-quality community-wide reference dataset.