Raw data for Figure 4A: Neutralising antibodies against IFN-γ and TNF-α reduce the immunosuppressive capacity of Mesoangioblasts/HIDEMs

CFSE labelled PBMCs were stimulated with anti-CD3/CD28 beads in the presence of HIDEMs/mesoangioblasts (1:4) and neutralising antibodies against IFN-γ and TNF-α or irrelevant isotype control antibody (0.5, 1.0 and 2.0 µg/ml) or recombinant IL-1RA (0.5, 1.0 and 2.0 µg/ml). Cells were harvested on day 6 and stained with anti-CD3 and 7AAD. After gating on CD3+7AAD- the number of CFSE diluting cells were enumerated using counting beads. Experiments were carried out in duplicates. n=4.

CFSE 标记的 PBMC 细胞在 HIDEMs/中胚层祖细胞（1:4）及针对 IFN-γ 和 TNF-α 的中和抗体或无关型同种异体控制抗体（0.5、1.0 和 2.0 µg/ml）或重组 IL-1RA（0.5、1.0 和 2.0 µg/ml）的存在下，经抗-CD3/CD28 球珠刺激。细胞于第 6 天收获并经抗-CD3 和 7AAD 染色。在 CD3+7AAD- 门控下，通过计数微球对 CFSE 稀释细胞的数量进行计数。实验重复进行。样本量 n=4。