Gene expression profile at single cell level from the whole brain of two tree shrew fetus

Although continual expansion of the brain during primate evolution accounts for our enhanced cognitive capabilities, the drivers of brain evolution have scarcely been explored. Tree shrews are closely related to primates that comparing their brains to primate brains at the single-cell level will provide new insights into the evolution of the primate brain. Whole brains of tree shrew embryos were collected from 2 two-thirds-gestation embryos (4-5 post-conceptional weeks) for single cell RNA-sequencing. The 10x library was constructed using 10x Chromium Controller and Chromium Single Cell 3’ Reagent Kits (v3 chemistry CG000183) following the detailed protocol of the manufacturer. Libraries were sequenced using an Illumina NovaSeq 6000 sequencer with 150-bp paired-end reads.