bacterial community of a polluted river by domestic water Raw sequence reads

The bacterial community in rivers receiving untreated domestic wastewater discharge may show specific longitudinal and vertical assemblage patterns due to wide gradients in physicochemical conditions shaped by periodic algal bloom. However, there remains a major lack of comprehensive understanding of such information. Accordingly, we used high-throughput sequencing of 16S rRNA gene amplicons and multivariate statistics to intensively characterize bacterial community composition across time and space in a river segment receiving domestic wastewater discharge in southeast China.