Candidate genes that modify mutant Nhtt(62Q)EGFP aggregation.

Following primary and secondary screening in Nhtt(62Q)EGFP and Nhtt(18Q)EGFP cells respectively, pruning for OTEs, and tertiary screening with de novo dsRNA probes in both cell lines, final candidates were placed into three groups. All candidates consistently altered mutant Htt aggregation in cell culture screening rounds. Group 1 candidates did not change the EGFP intensity of Nhtt(18Q)EGFP in the tertiary screen by more than 10%, group 2 by no more than 20% and group 3 by no more than 30%. The tertiary screen results for each candidate group are shown as the fold change in the number of inclusions (# inclusions), inclusion load and viability compared to a non-specific dsRNA control. Human and mouse homologues for each Drosophila candidate gene are listed.