Identification of ZEB2 binding sites in Hepatocellular Carcinoma Cell Line SNU398

ZEB2, a member of the ZEB family of EMT inducers, is involved in cellular proliferation, senescence, and invasion in most of the human cancers. However, genes regulated by ZEB2 in hepatocellular carcinoma (HCC) are not identified so far. Hence, we conducted a ChIP-Seq study in high ZEB2 expressing HCC cell line SNU398 by using a homemade anti-ZEB2 antibody (clone 6E5). Chromatin fragments bound by ZEB2 were subject to ChIP using ZEB2 antibody and followed by high-throughput sequencing