Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5'TOP mRNAs [4EBP1/2]

To investigate the changes in gene expression upon loss of 4EBP1/2, we performed RNA-seq and differential gene expression analysis on WT and derived 4EBP1/2 KD cell lines. The canonical and 5'TOP mRNA cell lines used for single-molecule imaging were treated as biological replicates for the differential gene expression analysis. Overall design: WT cells (canonical and 5'TOP mRNA cell lines) and derived 4EBP1/2 KD cell lines were analyzed by RNA-seq, using indepedent replicates (cells harvested on different days) run as paired-end reads.