Summary of FRET efficiency values for His-tagged GFP-RyR1 fusion constructs.
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aNumber of measurements.
bMean energy transfer values observed in the FRET experiments.
cFRET efficiency values predicted after docking the model comprised of the atomic structure of the N-terminal domain and triangulated GFPs to the cytoplasmic vestibule location in the cryo EM structure of RyR1 as indicated in Figure 6. The 2 sets of values for the GFP620 series of constructs represent predicted FRET to GFP620 placed at either of the two indicated positions shown in Figure 6.
创建时间：
2012-06-13
