Research data supporting GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth

This dataset corresponds to the final figures from ‘GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth’ by Jayne Griffiths, Annalisa Rizza, Bijun Tang, Wolf B. Fromer and Alexander M. Jones.
Data was collected at Sainsbury Laboratory, Cambridge University between approximately April 2019 and October 2023.
Any queries on the data can be emailed to Alexander M Jones ([e-mail]), the principal investigator on the project, or Jayne Griffiths ([e-mail]), the lead author.
Notes on the dataset:
All data is placed under a CC-BY-NC 4.0 International license.
Methods are detailed fully in the paper but include: Confocal microscopy, Advanced image analysis, Arabidopsis hypocotyl phenotyping, RT-qPCR. Microscopy data was analysed with Fiji and FRETENATOR1.5 as detailed in the paper.
Due to size constraints we have been unable to include all of our analysis but we have tried to include original image files as lif or tif files with excel files containing processed data. The metadata file includes the settings that were used to process the images using the FRETENATOR plugin in ImageJ (See software for link).
Whilst we have included all experiments and replicates that are included in the paper, data storage constraints meant we could not place our independent repeats of the experiments on this drive. These other replicates can be made available on request.
Image sequences from the IR rig are collected into .zip/.7z files which can be opened with a (de)compression program such as 7-zip. These files are organised by Figure.

本数据集对应于Jayne Griffiths、Annalisa Rizza、Bijun Tang、Wolf B. Fromer和Alexander M. Jones所著《GIBBERELLIN PERCEPTION SENSOR 2揭示光调控下细胞GA动态的起源与作用》一文的最终图表。数据收集于剑桥大学Sainsbury实验室，时间跨度约为2019年4月至2023年10月。有关数据的任何疑问，均可通过电子邮件联系项目负责人Alexander M. Jones（[e-mail]）或首席作者Jayne Griffiths（[e-mail]）。

数据集备注：
所有数据均置于CC-BY-NC 4.0国际许可之下。
研究方法详见论文，包括：共聚焦显微镜、高级图像分析、拟南芥下胚轴表型分析、实时定量PCR。显微镜数据使用Fiji和FRETENATOR1.5进行分析，具体方法见论文。
由于容量限制，我们未能包含所有分析数据，但已尽可能包括原始图像文件（lif或tif格式）以及包含处理数据的Excel文件。元数据文件包含了使用ImageJ中的FRETENATOR插件处理图像时所使用的设置（参见软件链接）。
尽管我们已包括论文中提到的所有实验和重复次数，但数据存储限制导致我们无法在此驱动器上放置独立重复的实验。这些其他重复实验可根据请求提供。
红外装置的图像序列收集于.zip/.7z文件中，可使用7-zip等（解）压缩程序打开。这些文件按图表进行组织。