Supplemental Table S5

RNA-seq was performed using RNA isolated from non-injured and skeletal muscle at 5 days post-injury (dpi) of wt and <i>ankrd1a </i>mutant fish (four fish per group). Differential expression was calculated using a multi-factorial statistical analysis based on a negative binomial model that used a generalized linear model approach computed by edgeR (RRID:SCR_012802) from raw counts in each comparison (16 samples in 4 different groups). Multiple testing controlling procedure was applied and genes with an FDR ≤ 0.05 and logFC &gt; |0.5| were considered differentially expressed. Annotation of differentially expressed genes was performed using the bioMart package (v2.60.1) into R environment (v4.3.3), querying available Ensembl Gene IDs and retrieving Gene Names and Entrez gene IDs. The complete RNA-seq data have been deposited in NCBI´s Gene Expression Omnibus (RRID:SCR_005012) and are accessible through GEO Series accession number GSE277480 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277480).