Measurement of reduced, oxidized (dehydroascorbate) and total ascorbate content in cucumber seedlings and the ASC/DHA ration

Cotyledons were sampled from seven days old seedlings grown in soil with or without Trichoderma (Control and +T203) with or without 75 mM NaCl (+NaCl and +T203 +NaCl). Quantification of the reduced (AA) and oxidized (DHA) forms of ascorbate in plants was done as describe in material and method. The ratio between the reduced and oxidized form of ascorbic acid is shown (AA/DHA). Results are average of three independent pools of plant material ± standard deviation. Statistically significant differences between treatments (P<0.05) are marked with distinct letters (A,B,C,D) for AA, DHA and AA/DHA.