Transcriptomic analysis of adhesive capsulitis of the shoulder

We compared genes from tissue of patients with adhesive capsulitis (AC) with those having surgery for shoulder instability to determine potential biomarkers specific to AC through transcriptomic analysis. Our results presented increased expression of PDGFB, COL18A1 and MMP9 in patients with AC, while TNFA expression was reduced. Overall design: Tissue samples were collected from the anterior capsule and rotator interval during surgery. Total RNA was extracted and RNA-sequencing-based transcriptomics were performed. Bioinformatic analyses were carried out to identify biological processes and pathways enriched in this dataset. A number of genes deemed differentially expressed in RNA-sequencing analysis were validated using real time RT-PCR.