Tetrastigma hemsleyanum cultivar:Jinhulu2 Genome sequencing and assembly

Tetratigma hemsleyanum Diels & Gilg is a well-known traditional Chinese medicine, renowned for its remarkable medicinal value in various clinical contexts. The corresponding chloroplast and nuclear genomes have since been sequenced, providing valuable information for breeding and exploration. However, the mitochondrial genome of T. hemsleyanum remains uncharacterized, which restricts a broad view of its evolution and utilization.In this study, we assembled the mitochondrial genome of T. hemsleyanum var. Jinhulu 2 using a combined approach of Illumina and Nanopore sequencing technologies, followed by a comparative analysis with its close relatives. The mitogenome features a multi-chromosomal structure, comprising two circular chromosomes with a total length of 809,173 bp and a GC content of 44.35%. It contains 76 annotated functional genes, including 35 protein-coding genes, 37 tRNA genes, 3 rRNA genes, and 1 pseudogene. Analysis revealed 273 simple sequence repeats, 109 tandem repeats, and 8,733 dispersed repeats, suggesting a higher repeat abundance in T. hemsleyanum relative to related species.