Cell-sheet shape transformation by internally-driven, oriented forces

During morphogenesis, cells collectively execute directional forces that drive the programmed folding and growth of the layers, forming tissues and organs. The ability to recapitulate aspects of these processes in vitro will constitute a significant leap forward in the field of tissue engineering. Free-standing, self-organizing, cell-laden matrices are fabricated using a sequential deposition approach that uses liquid crystal-templated hydrogel fibers to direct cell arrangements. The orientation of hydrogel fibers is controlled using flow or boundary cues, while their microstructures are controlled by depletion interaction and probed by scattering and microscopy. These fibers effectively direct cells embedded in a collagen matrix, creating multilayer structures through contact guidance and by leveraging steric interactions amongst the cells. In uniformly aligned cell matrices, oriented cells exert traction forces that can induce preferential contraction of the matrix. Simultaneously, th..., , # Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces

Dataset DOI: [10.5061/dryad.8gtht770q](10.5061/dryad.8gtht770q)

## Description of the data and file structure

Title of Dataset: Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces

This dataset contains the data presented in the main text figures and supplemental information of \"Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces\", by authors Junrou Huang, Juan Chen, and Yimin Luo in Advanced Material. (Journal citation: J. Huang, J. Chen, Y. Luo, Adv. Mater., 2025, 2416624. [http://doi.org/10.1002/adma.202416624](http://doi.org/10.1002/adma.202416624)).

Descriptions of the data and file structure:

Data are all provided in comma separated value form (CSV). File names refer to the figures numbers of the manuscript. Processing scripts and image analyzed are also uploaded. If the letter S is provided before the number, the figure can be found in the Supporting Information.

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