Genetic profile of HaCaT Cells stimulated by AD skin derived Staphylococcus aureus and Cytokines

The genetic profile of HaCaT cells was found to be greatly altered by S. aureus stimulation from the control, and the addition of cytokines changed the genetic profile to a different one from only S. aureus stimulation. We picked up genes that have been reported to be dominantly involved in AD, innate immunity, and other related things and checked the difference between their expression by HaCaT cells treated with cytokines and S. aureus and those without the treatments. In S. aureus stimulation, the expression of IL1a, IL8R1, TLR1, TLR6, FN1, FLG, and TSLP was increased compared with the control of HaCaT cells. In AD strain (TF3378) stimulation, the expression of IL1b, IL6, and ITGa5 was increased compared with standard strain. IFN-gamma upregulated the expression of IL12, IL8R1, and ITGa5 but suppressed the expression of IL13RA2, FN1, and FLG. While on the other hand, IL-4 stimulation upregulated IL13RA2, FLG, and TSLP, downregulated FN1, and IL33.