CAST/EiJ x C57BL/6J cross data

Here we introduce a test for lineage-specific selection of cis-regulation that can be applied to any gene set of interest. Applying the test to hundreds of gene sets in two diverged subspecies of mouse, we find evidence for selection acting on the cis-regulation of several functional classes, including those involved in both physiology (e.g. mitochondria-related genes and growth regulators) and behavior (e.g. locomotory-related genes and memory-related genes). These positively selected gene sets implicate potential targets of positive selection that are supported by quantitative trait loci, and accurately predict a number of phenotypic differences between the two subspecies, suggesting that the divergence in these traits may have been driven by adaptive evolution. Dual-channel ratio data for 23,653 genes across 3 tissues in 443 CASTxB6 F2 mice NO RAW DATA HAS BEEN SUBMITTED TO GEO AT THIS TIME. THIS SUBMISSION WAS APPROVED AT THE REQUEST OF THE SUBMITTER TO ALLOW PUBLICATION REVIEWER ACCESS.