Proteomics in Endurance Athletes with Atrial Fibrillation

Aptamer-Based Proteomics Profiling: The modified aptamer binding reagents, SOMAscan assay technique, performance characteristics, and analysis have previously been described. In brief, protein levels for ~7,000 analytes (Supplementary Table II) in the archived dipotassium ethylenediaminetetraacetic acid plasma samples were measured by the SOMAscan platform, which uses single‐stranded DNA‐based aptamers to translate protein concentrations into DNA signals measurable by standard DNA detection methodologies. Plasma samples had a single controlled freeze-thaw cycle on ice prior to proteomics profiling which was required for aliquoting and shipping purposes. Target annotation and mapping of aptamers to UniProt accession numbers as well as Entrez gene identifiers were provided by SomaLogic; normalization information has been provided in the associated manuscript. The associated proteomics dataset can be requested from the corresponding authors, which, following institutional data sharing approval, is analyzable using SOMALogic's analysis suite (https://stats.somalogic.com/studyqc).