Effect of GITR on MM tumor proliferation in vitro and in vivo.

A) MM cells (MM.1S and OPM1) were transfected with PCDH empty and PCDH-GITR with GFP labeled lentiviral vector (Cat# CD511B-1, SBI Inc.) respectively. Cell proliferation has been evaluated by using GFP competition assay. Expression of GFP and GITR has been examined by flow cytometry by using anti-human GITR PE labeled primary antobidy. GFP+ and GFP-MM cell were sorted by BD laser II flow machine. Ratio of GFP+/GFP-was recorded everyday after combination. B) Effect of GITR-overexpression on MM1.S cell line proliferation. 96 wells plate coated with MM1.S cells were read after 48 and 72 hours respectively. Anti-Brdu-pod was used to detect the absorbance. Mean±SD. *, P