James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12373, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Movie showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for this movie is included in this grouped set.

本数据集包含一幅描绘鹤虻（Nephrotoma suturalis）初级精母细胞中动粒微管在减数分裂I期动态变化的电影。该电影通过使用尼康Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi公司，位于马萨诸塞州Woburn），配备60倍/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜和2.0倍变焦镜头进行时间推移偏振显微镜拍摄而成。图像以每15秒一帧的频率由QImaging Retigo EXi CCD相机捕捉。原始图像经过Shribak和Oldenbourg（2003）提出的5帧算法处理。用于制作该电影的时序数据包含在本数据集集中。