Raw Nanopore sequencing data of CD-pore-seq V1: pol-free CD pore seq raw

=Raw Nanopore sequencing data of CD-pore-seq V1=

==Author==Zicheng Yu1, Zihao Chen1, Thomas C. Williams1,2, Craig J. Anderson1,3,4, Andrew P. Jackson1,*, Martin A.M. Reijns1,*, Martin S. Taylor1,*1 MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, UK.2 Child Life and Health, University of Edinburgh, UK.3 German Cancer Research Center, Heidelberg, Germany4 Department of Translational Oncology, German Cancer Research Center, Munich, Germany.

* Correspondence should be addressed to [e-mail], [e-mail] and [e-mail]

==Citation=='Generalised training for DNA modification detection by nanopore sequencing'

==Description==Nanopore sequencing data of libraries to train machine learning models to predict single DNA-embedded ribonucleotides.Ligation kit: LSK-114 (all except for ygDNA) or SQK-NBD114.96 (ygDNA)Flow-cell version: R10.4.1Sequencing device: MinION or PromethION (./TelN/PromethION only)

==pol-free_CD_pore_seq==* Sequencing a pilot testing library on a MinION flow-cell, prepared by polymerase-free CD-pore-seq using synthetic oligos.* Directly ligation of three hybrids: Adapter_USER; Adapter_TelN; test oligo (1:1:1).* Library preparation: DIG-immobilisation using protein-G dynabeads -> USER digestion -> Biotin-immobilisation using strep-M280 dynabeads -> TelN digestion -> T4-PNK end-prep -> Adapter Ligation.