Microscopic rate constants for MaPgb* from the fit of the flash photolysis data, at 20°C.

Activation free energies were estimated from the linear Eyring plots for each rate constant ki in the temperature range 10–40°C.*The value of these rates and their associated temperature dependence are difficult to estimate given the small amplitude of the processes.**the present analysis did not allow to appreciate a difference in kout for the two conformations.The numerical reliability of each parameter was defined by evaluating the dependence of the fitness function on changes of the parameter, assuming the others as constant. k−1≈; k2 , k−2≈10%; k3 , k−3≈10%.The rate equations for CO binding are very poorly sentitive to kd,r and kd,t, which are held constant to the values determined by the following equation: kOFF,1/2 = kd,r/t×kout/(kout+kg,r/t).